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KMID : 1013220230210020081
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Jouranl of the Korean Society of Clinical Toxicology
2023 Volume.21 No. 2 p.81 ~ p.91
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Protective effects of mealworm (Tenebrio molitor) extract on N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)?induced cellular toxicity in SH-SY5Y neuroblastoma cells
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Jo In-Ho
Kim Yoo-Ji
Kim Seon-Tae
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Abstract
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Purpose: Edible insect extracts have been used as an alternative source for medicinal supplements due totheir significant antioxidative and anti-inflammatory activity. Recent studies have reported that anti-mi crobial peptides from insects have neuroprotective effects on dopamine toxins. The purpose of this studywas to investigate the protective functions of mealworm (Tenebrio molitor) extract (MWE) on N-meth yl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)?induced cellular toxicity in SH-SY5Y neuroblastoma cells.
Methods: Cellular toxicity induced by the MPTP toxin and the impact of MWE on cell survival were ana lyzed using MTT assays. DAPI staining was performed to observe apoptotic phenomena caused by MPTP.
Changes in caspase-3 activity and protein expression were observed using enzyme activity assays andwestern blot assays, respectively.
Results: MWE exerted significant antioxidant activity, which was measured by both DPPH and ABTS radi cal assays, with a dose-dependent relationship. Furthermore, MWE resulted in cellular proliferation in SH SY5Y cells in a dose-dependent manner. Furthermore, MWE pretreatment significantly inhibitedMPTP-induced cytotoxicity, with a dose-dependent relationship. The morphological characteristics ofapoptosis and increased reactive oxygen species induced by MPTP were also significantly reduced byMWE pretreatment.
Conclusion: MWE treatment significantly attenuated MPTP-induced changes in the levels of proteins asso ciated with apoptosis, such as caspase-3 and PARP. These findings suggest that MWE exerts neuroprotectiveeffects on human neuroblastoma SH-SY5Y cells subject to MPTP-induced dopaminergic neurodegeneration.
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KEYWORD
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SH-SY5Y cells, MPTP, Mealworm extract, Antioxidative, Anti-apoptosis
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